[[Anchor(#Tissue Culture Tasks)]]

== Tissue Culture Tasks ==
||Tayo ||Water Bath and Sonicator ||Check weekly for sufficient water levels and for contamination. Remove all water, clean with IPA and replace water along with antibiotics at least once/month. ||
||Person needed ||CO2 Tanks ||Check the CO2 tanks weekly on Friday. Order new tanks and change over when necessary. ||
||Person needed ||Incubator ||Check weekly for unlabeled cultures, old cultures (>2 weeks) and cultures with visible contamination. Also change water in water pans (with DI water) and make sure incubator is not alarming. Move everything to other incubator, wipe down with IPA and change water pans. Wait an hour for vapor to dissipate before moving everything back. Change filter when necessary. ||
||Person needed ||Solutions ||Check weekly and make 70% Ethanol, 70% IPA, 10% Bleach and 7x cleaning solution and fill all squirt/spray bottles when necessary. ||
||Person needed ||Frozen Aliquots ||Check weekly, order and aliquot HS, FBS and Pen/Strep when necessary. Make up plating media for initial dilution of neonatal rat cardiomyocyte isolation. ||
||Kevin Chung ||Waste disposal ||Check boxes and sharps container weekly. Close up the sharps box and the Pasteur pipette jug and replace when necessary. ||
||Dan ||Sterilization ||Run all items in the sterilizing bin through the sterilizer weekly. Ensure neonate cardiomyocyte isolation stuff if sterilized by day of isolation ||
||Person needed || Supplies ||Check weekly on the level of 1ml, 2ml, 5ml, 10ml and 25ml pipettes, 15ml and 50ml centrifuge tubes,  20ul, 200ul and 1ml pipette tips and 6-well plates. Make sure drawers and shelves are full in the TC room and make sure more get ordered if needed. ||
||Atsu ||Glassware ||Weekly put away drying glassware and put down new bench paper in the drying area. Generally clean sink area. ||
||Person needed ||Refrigerator ||Weekly check through the refrigerator for old or unlabeled items. Everything should be labeled with a name and date at the minimum. ||

[[Anchor(Cell Culture Schedule)]]

== Cell Culture Schedule ==
=== June 24 ===
20 rats ordered

 * Atsu -- 20 million cells
 * Amy -- 3 million cells
=== July 3 ===
 * Karen -- 15 million cells

=== July 10 ===
 * Karen -- 20 million cells

=== July 17 ===
 * Karen -- 20 million cells
 * Jennifer -- 70 million cells

=== July 24 ===
 * Joyce-- 10 million cells
 * Karen -- 40 million cells

=== July 29 ===
 * Jen -- 10 million cells
 * Joyce -- 10 million cells
 * Jessica -- 50 million cells


=== July 30 ===
 * Karen -- 40 million cells


=== August 4 ===
 * Jessica -- 40 million cells

=== August 5 ===
 * Jen S. -- 20 million cells

=== August 6 ===
 * Karen -- 40 million cells

=== August 12 ===
 * Joyce -- 30 million cells

=== August 12 ===
 * Jen/Rob -- 20 million cells

=== August 11 ===
 * Jessica -- 40 million cells

=== August 14 ===
 * Karen -- 20 million cells

=== August 19 ===
 * Joyce -- 15 million cells
 * Jen/Rob -- 15 million cells

=== August 26 ===
 * Joyce/Dr. Yang -- 30 million cells
 * Jessica -- 40 million cells

=== September 2 ===
 * Joyce -- 15 million cells

=== September 9 ===
 * Dr.Yang -- 15 million cells

=== September 16 ===
 * Jessica -- 40 million cells
 * Dr.Yang -- 15 million cells

=== September 23 ===
 * Atsu -- 30 million cells
 * Dr.Yang --21 million cells
 * Joyce -- 10 million cells

=== October 3 ===
 * Atsu -- 30 million cells

=== October 10 ===
 * Barbara -- 10 million cells
 * Atsu -- 30 million cells


=== October 17 ===
 * Atsu -- 40 million cells
 * joyce -- 10 million cells

=== October 24 ===
 * Barbara -- 10 million cells
 

=== October 31 ===
 * Joyce -- 15 million cells
 
=== November 14 ===
 * Barbara -- 20 million cells

=== December 5 ===
 * Barbara -- 30 million cells

=== December 12 ===
 * Barbara -- 30 million cells

=== January 23 ===
 * Barbara -- 42 million cells

=== February 6 ===
 * Barbara -- 28 million cells
 * Atsu -- any extra cells

=== February 13 ===
 * Atsu -- 40 million cells

=== February 27 ===
 * Barbara -- 21 million cells

=== March 6 ===
 * Joyce -- 15 million cells

=== March 13 ===
 * Barbara -- 12 million cells

=== March 20 ===
 * Barbara -- 12 million cells

=== March 27 ===
 * Barbara -- 10 million cells

=== April 30 ===
 * Barbara -- 10 million cells

=== May 5 ===
 * Joyce -- 10 million cells